| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)
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| Autores: |
Bado Nilles, A
Gagnaire, Beatrice
Thomas-guyon, Helene
Le Floch, S
Renault, Tristan
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| Data: |
2008-09
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| Ano: |
2008
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| Palavras-chave: |
Toxicity
PAHs
Pacific oyster
Spectrophotometry
Flow cytometry
Haemocyte activity
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| Resumo: |
The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. (C) 2008 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2008/publication-4696.pdf
DOI:10.1016/j.tiv.2008.04.011
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/4696/
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| Formato: |
application/pdf
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| Fonte: |
Toxicology in Vitro (0887-2333) (Elsevier), 2008-09 , Vol. 22 , N. 6 , P. 1610-1617
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| Direitos: |
2008 Elsevier Ltd All rights reserved.
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